| 研究背景 | Tumor necrosis factor Receptor superfamily member 3, also known as Lymphotoxin-beta Receptor,Tumor necrosis factor C Receptor,Tumor necrosis factor Receptor 2-related Protein,Tumor necrosis factor Receptor type III,LTBR,TNFCR, TNFR3 and TNFRSF3, is a member of the tumor necrosis factor (TNF) family of Receptors. LTBR is a single-pass type I membrane Protein and contains four TNFR-Cys repeats. It is expressed on the surface of most cell types, but not on T and B lymphocytes. LTBR and its Ligand play a role in the development and organization of lymphoid tissue and transformed cells. Activation of LTBR can trigger apoptosis. In addition, LTBR can lead to the release of the Cytokine Interleukin 8. |
|---|---|
| 种属 | 人 |
| 表达系统 | Human Cells |
| 标签 | C-Fc |
| 蛋白编号 | P36941 |
| 别名 | Tumor Necrosis Factor Receptor Superfamily Member 3;Lymphotoxin-Beta Receptor;Tumor Necrosis Factor C Receptor;Tumor Necrosis Factor Receptor 2-Related Protein;Tumor Necrosis Factor Receptor Type III;TNF-RIII;TNFR-III;LTBR;D12S370;TNFCR |
| 氨基酸序列 | Gln31-Met227 |
| 分子量 | 48.8 kDa（observed：61 kDa） |
| 纯度 | > 95% as determined by reducing SDS-PAGE. |
| 性状 | 粉末 |
| 组分 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 内毒素含量 | < 0.1 EU/μg |
| 复溶方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 保存条件/期限 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输条件 | Ambient temperature or ice pack. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
