PeproGMP® Human BMP-4 Recombinant Protein

说明书

种属：

Human

表达系统：

E. coli

货号：TS428765

价格：

货期：

现货

规格：

数量：

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商品详情

产品信息

描述	The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
研究背景	Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-beta superfamily of structurally related signaling proteins. PeproTech's E. coli-derived Human BMP-4 is a fully active homodimeric protein consisting of two 107 amino acid subunits, which correspond to amino acids 303-408 of the full-length BMP-4 precursor, as well as an N-terminal methionine. PeproTech Recombinant Human BMP-4 is a 24.2 kDa protein.PeproGMP BMP-4 can be used withPeproGMP VEGF-165andPeproGMP SCFinCTS StemScale mediumfor pluripotent stem cell differentiation, specifically for mesoderm and hematopoetic progenitor cell induction.Reconstitute using 5mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/mL.Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.AllPeproGMP®products are manufactured using animal-derived component free materials.Product Description/Intended Use:PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
生物活性	Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
种属	Human
表达系统	E. coli
蛋白编号	(Human) P12644
偶联	UnconjugatedUnconjugatedUnconjugated
中文名	BMP; Bone morphogenetic protein; bone morphogenetic protein 2B
别名	BMP2B; BMP2B1; MCOPS6; OFC11; ZYME
氨基酸序列	MKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
分子量	24.2 kDa
纯度	≥ 98% by SDS-PAGE gel and HPLC analyses.
性状	Lyophilized
内毒素含量	≤0.1 EU/µg
保存条件/期限	-20°C or -80°C if preferred
运输条件	Ambient

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

计算重置

起始浓度（M）× 起始体积（L）= 最终浓度（M）× 最终体积（L）

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

计算重置

分子量计算器

Enter the chemical formula of the compound to calculate its molar mass and elemental composition

分子结构式

总分子量

g/mol

计算重置

动物实验计算换算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH₂O，那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 100 μL DMSO （母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法： 取 100 μL DMSO 母液，添加 400 μL PEG300 混匀澄清，再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH₂O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD，玉米油等，均可在诺渊网站点击购买。

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% Saline/PBS/ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。