Human LIGHT Recombinant Protein

说明书

种属：

Human

表达系统：

Insect cells

货号：TS428767

价格：

货期：

现货

规格：

数量：

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商品详情

产品信息

描述	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
研究背景	310-09B-100UG will be provided as 15 µg (310-09B-15UG) vials.310-09B-1MG will be provided as 15 µg (310-09B-15UG) vials.310-09B-250UG will be provided as 15 µg (310-09B-15UG) vials.310-09B-500UG will be provided as 15 µg (310-09B-15UG) vials.Recombinant Human LIGHT has a calculated mass of 19.3 kDa, containing 177 amino acid residues. Due to glycosylation, LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions. *Human LIGHT(Catalog Number 310-09B) has replaced Human LIGHT(Catalog Number 310-09)This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
生物活性	Determined by its ability to induce IL-8 secretion in human BEAS-2B cells.
种属	Human
表达系统	Insect cells
蛋白编号	(Human) O43557
偶联	UnconjugatedUnconjugatedUnconjugated
中文名	herpesvirus entry mediator ligand; HVEM-L; TNF homolog; sCD258; sLIGHT; soluble CD258; soluble LIGHT; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; tumor necrosis factor superfamily member 14
别名	CD258; HVEML; LIGHT; LTg
氨基酸序列	RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV
分子量	19.3 kDa
纯度	≥ 95% by SDS-PAGE gel and HPLC analyses.
性状	Lyophilized
内毒素含量	<1 EU/µg
保存条件/期限	-20°C
运输条件	Ambient

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

计算重置

起始浓度（M）× 起始体积（L）= 最终浓度（M）× 最终体积（L）

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

计算重置

分子量计算器

Enter the chemical formula of the compound to calculate its molar mass and elemental composition

分子结构式

总分子量

g/mol

计算重置

动物实验计算换算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH₂O，那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 100 μL DMSO （母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法： 取 100 μL DMSO 母液，添加 400 μL PEG300 混匀澄清，再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH₂O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD，玉米油等，均可在诺渊网站点击购买。

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% Saline/PBS/ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。