Immunohistochemical
Human lung carcinomaPrimary Antibody Dilution :1:300Secondary Antibody :Anti-rabbit-linker, Fbex-HRPSecondary Antibody Dilution :NOT FOUNDColor/Signal Descriptions :Brown: IGFBP1 Blue: NucleiGene Name :IGF2BP1Submitted by :Haodong Xu. Department of Pathology, University of Rochester medical CenterWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | The immunogen for anti-IGF2BP1 antibody: synthetic peptide directed towards the N terminal region of human IGF2BP1 |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | WB |
| 物种反应性 | BovineDogGuinea PigHorseHumanPigRat |
| 浓度 | 1.0mg/ml |
| 纯化 | Affinity purified |
| 基因名称 | IGF2BP1 |
| 基因别名 | CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1 |
| 基因全称 | Insulin-like growth factor 2 mRNA binding protein 1 |
| 基因简介 | IGF2BP1 is a member of the IGF-II mRNA-binding protein (IMP) family. The protein contains four K homology domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation.This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| 蛋白别名 | CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1 |
| 分子量 | 63kDa |
| 序列 | 577 amino acids |
| Gene ID | 10642 |
| Uniprot ID | Q9NZI8 |
| 应用稀释比 |
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| 修饰 | Unmodification |
| 标记 | Unconjugated |
| 产品形态 | Liquid |
| 缓冲液 | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
