| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | L-aspartic acid-gluteraldehyde-albumin conjugate |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | ELISAIHC-FIHC-P |
| 物种反应性 | HumanOther |
| 浓度 | 1.0mg/ml |
| 纯化 | Affinity purified |
| 基因别名 | Aspartate antibody Aspartic acid antibody L ASP antibody L aspartate antibody |
| 靶标 | Aspartate |
| 应用稀释比 |
For ELISA, IHC-Fr and -P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| 产品详情 | Rabbit polyclonal to AspartateThe antiserum was tested using the free floating PAP technique on rat cortex. The anti-conjugated Aspartate antibodies gave good staining between 1/2000-1/5000 dilution in these areas.Aspartic acid (Asp), also known as aspartate, is one of the 20 natural proteinogenic amino acids which are the building blocks of proteins. It is non-essential in mammals, and might serve as an excitatory neurotransmitter in the brain. It is also a metabolite in the urea cycle and participates in gluconeogenesis. As a neurotransmitter, aspartic acid may provide resistance to fatigue and thus lead to endurance, although the evidence to support this idea is not strong. |
| 产品形态 | Liquid |
| 缓冲液 | Antiserum containing no perservatives |
| 保存条件 | Prior to reconstitution store at 4°C. Following reconstitution keep as concentrated solution, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
