JMJD6 Recombinant Rabbit Monoclonal Antibody

说明书

宿主：

Rabbit

已测试的应用：

ChIP,FC,ICC/IF,IHC,IP,WB

物种反应性：

Human,Monkey,Mouse,Rat

货号：TS763505

价格：

货期：

现货

规格：

数量：

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商品详情

产品信息

抗体类型	Primary Antibodies
免疫原	Recombinant protein within human JMJD6 aa 1-360.
克隆性	Monoclonal
同种型	IgG
宿主	Rabbit
已测试的应用	ChIPFCICC/IFIHCIPWB
物种反应性	HumanMonkeyMouseRat
浓度	1mg/ml
纯化	Protein A
基因名称	JMJD6
基因别名	PSR; PTDSR; PTDSR1
基因全称	jumonji domain containing 6, arginine demethylase and lysine hydroxylase
基因简介	This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
蛋白别名	Apoptotic cell clearance receptor antibody;Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody;Histone arginine demethylase JMJD6 antibody;JmjC domain-containing protein 6 antibody;JMJD 6 antibody;JMJD6 antibody;JMJD6_HUMAN antibody;Jumonji domain containing 6 antibody;Jumonji domain-containing protein 6 antibody;KIAA0585 antibody;Apoptotic cell clearance receptor antibody;Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody;Histone arginine demethylase JMJD6 antibody;JmjC domain-containing protein 6 antibody;JMJD 6 antibody;JMJD6 antibody;JMJD6_HUMAN antibody;Jumonji domain containing 6 antibody;Jumonji domain-containing protein 6 antibody;KIAA0585 antibody;Lysyl-hydroxylase JMJD6 antibody;Peptide-lysine 5-dioxygenase JMJD6 antibody;Phosphatidylserine receptor antibody;Protein PTDSR antibody;PSR antibody;PTDSR 1 antibody;PTDSR antibody;PTDSR1 antibody
分子量	46kDa(Observed band size: 50/110-260kDa)
应用稀释比	WB:1:5000 IHC:1:8000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample ChIP:Use 5 μg for 25 μg of chromatin.
标记	Unconjugated
亚细胞定位	Nucleus, nucleoplasm, nucleolus, Cytoplasm.
产品形态	Liquid
缓冲液	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Western blot analysis of JMJD6 on different lysates with Rabbit anti-JMJD6 antibody at 1/5,000 dilution.Lane 1: 293T cell lysate,Lane 2: PANC-1 cell lysate,Lane 3: HeLa cell lysate,Lane 4: SH-SY5Y cell lysate,Lane 5: COS-1 cell lysate,Lane 6: Neuro-2a cell lysate,Lane 7: PC-12 cell lysate,Lysates/proteins at 20 µg/Lane.Exposure time: 2 minutes; 4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

IHC

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-JMJD6 antibody at 1/8,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ICC/IF

Immunocytochemistry analysis of PC-12 cells labeling JMJD6 with Rabbit anti-JMJD6 antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JMJD6 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of PC-12 cells labeling JMJD6.Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

JMJD6 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-JMJD6 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-JMJD6 antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: HeLa cell lysate (input),Lane 2: Rabbit anti-JMJD6 antibody IP in HeLa cell lysate,Lane 3: Rabbit IgG instead of Rabbit anti-JMJD6 antibody in HeLa cell lysate,Blocking/Dilution buffer: primary antibody dilution,Exposure time: 3 minutes.

ChIP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with JMJD6 or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

计算重置

起始浓度（M）× 起始体积（L）= 最终浓度（M）× 最终体积（L）

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

计算重置

分子量计算器

Enter the chemical formula of the compound to calculate its molar mass and elemental composition

分子结构式

总分子量

g/mol

计算重置

动物实验计算换算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH₂O，那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 100 μL DMSO （母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法： 取 100 μL DMSO 母液，添加 400 μL PEG300 混匀澄清，再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH₂O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD，玉米油等，均可在诺渊网站点击购买。

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% Saline/PBS/ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

JMJD6 Recombinant Rabbit Monoclonal Antibody

产品信息

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

起始浓度（M）× 起始体积（L）= 最终浓度（M）× 最终体积（L）

分子量计算器

动物实验计算换算器

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