WB
Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HepG2 cells labeling PAR2.| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Synthetic peptide within Human PAR2 aa 328-377 / 397. |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | FCWB |
| 物种反应性 | HumanMouse |
| 浓度 | 1mg/ml |
| 纯化 | Protein A |
| 基因名称 | F2RL1 |
| 基因别名 | PAR2; GPR11 |
| 基因全称 | F2R like trypsin receptor 1 |
| 基因简介 | This gene encodes a member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity. [provided by RefSeq, Jun 2016] |
| 蛋白别名 | Coagulation factor II receptor like 1 antibody; Coagulation factor II receptor-like 1 antibody; Coagulation factor II thrombin receptor like 1 antibody; F2RL1 antibody; G protein coupled receptor 11 antibody; G-protein coupled receptor 11 antibody; GPR11 antibody; PAR 2 antibody; PAR-2 antibody; PAR2_HUMAN antibody; Protease activated receptor 2 antibody; Proteinase activated receptor 2 antibody; Proteinase-activated receptor 2 antibody; Thrombin receptor like 1 antibody; Thrombin receptor-like 1 antibody |
| 分子量 | 44 kDa(Observed band size: 55 kDa) |
| 应用稀释比 |
WB:1:1000 FC:1:500-1:1000 |
| 亚细胞定位 | Cell membrane. |
| 产品形态 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| 保存条件 | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
