WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TTF1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Rat lung was stained with anti-TTF1 rabbit antibody.IHC
Mouse lung was stained with anti-TTF1 rabbit antibody.IHC
Human lung adenocarcinoma was stained with anti-TTF-1 Rabbit antibody.IF-P
Immunofluorescence multiplex immunohistochemical analysis of Rat lung (formalin-fixed paraffin-embedded section). using 488(Green) fluorescent tyramide signal amplification system. Antigen retrieval (pH 9.0, 20 minutes) was used before antibody incubation. DAPI (dark blue) was used as a nuclear counter stain.| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | The specific immunogen used to produce this antibody is proprietary information. |
| 克隆性 | Monoclonal |
| 同种型 | IgG,K |
| 宿主 | Rabbit |
| 已测试的应用 | IF-PIHCWB |
| 物种反应性 | HumanMouseRat |
| 浓度 | 1mg/ml |
| 纯化 | Protein A |
| 基因名称 | NKX2-1 |
| 基因别名 | BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1 |
| 基因全称 | NK2 homeobox 1 |
| 基因简介 | This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014] |
| 蛋白别名 | NKX2-1 ; NKX2A ; TITF1 ; TTF1 ; Homeobox protein Nkx-2.1 ; Homeobox protein NK-2 homolog A ; Thyroid nuclear factor 1 ; Thyroid transcription factor 1 ; TTF-1 |
| 分子量 | 40 kDa |
| 应用稀释比 |
WB:1:2000-1:10000 IHC:1:200-1:1000 IF-P:1:200-1:1000 |
| 亚细胞定位 | Nuclear. |
| 产品形态 | Liquid |
| 缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
| 保存条件 | -15°C to -25°C/1 year (Do not lower than -25°C). |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
