WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LC3B antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Mouse brain was stained with anti-LC3B rabbit antibody.IHC
Rat brain was stained with anti-LC3B rabbit antibody.IHC
Human brain was stained with anti-LC3B rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Histone H3 Antibody (red) was diluted at 1:200 (4℃ overnight).LC3B Rabbit mAb (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).IF-P
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | The specific immunogen used to produce this antibody is proprietary information. |
| 克隆性 | Monoclonal |
| 同种型 | IgG,K |
| 宿主 | Rabbit |
| 已测试的应用 | ICC/IFIF-PIHCWB |
| 物种反应性 | HumanMousePigRat |
| 浓度 | 1mg/ml |
| 纯化 | Protein A |
| 基因名称 | MAP1LC3B |
| 基因别名 | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| 基因全称 | microtubule associated protein 1 light chain 3 beta |
| 基因简介 | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| 蛋白别名 | MAP1LC3B |
| 分子量 | 14 kDa,16 kDa |
| 应用稀释比 |
WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
| 亚细胞定位 | Cytoplasm. |
| 产品形态 | Liquid |
| 缓冲液 | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
| 保存条件 | -15°C to -25°C/1 year (Do not lower than -25°C). |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
