SSRP1 Recombinant Rabbit Monoclonal Antibody

说明书

宿主：

Rabbit

已测试的应用：

IHC,WB

物种反应性：

Human,Monkey,Mouse,Rat

货号：TS774367

价格：

货期：

现货

规格：

数量：

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商品详情

产品信息

抗体类型	Primary Antibodies
免疫原	Recombinant protein within human SSRP1 aa 1-500.
克隆性	Monoclonal
同种型	IgG
宿主	Rabbit
已测试的应用	IHCWB
物种反应性	HumanMonkeyMouseRat
浓度	1mg/ml
纯化	Protein A
基因名称	SSRP1
基因别名	FACT; T160; FACT80
基因全称	structure specific recognition protein 1
基因简介	The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]
蛋白别名	Chromatin-specific transcription elongation factor 80 kDa subunit antibody; Cisplatin DNA SSRP antibody; Facilitates chromatin remodeling 80 kDa subunit antibody; Facilitates chromatin transcription complex 80 kDa subunit antibody; Facilitates chromatin transcription complex subunit SSRP1 antibody; FACT 80 kDa subunit antibody; FACT complex subunit SSRP1 antibody; FACT80 antibody; FACTp80 antibody; hSSRP1 antibody; Recombination signal sequence recognition protein 1 antibody; Recombination signal sequence recognition protein antibody; SSRP 1 antibody; SSRP1 antibody; SSRP1_HUMAN antibody; Structure specific recognition protein 1 antibody; Structure-specific recognition protein 1 antibody; T160 antibody
分子量	81 kDa
应用稀释比	WB:1:2000 IHC:1:200-1:1000
亚细胞定位	Nucleus, nucleolus, Chromosome.
产品形态	Liquid
缓冲液	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Western blot analysis of SSRP1 on different lysates with Rabbit anti-SSRP1 antibody at 1/2,000 dilution.Exposure time: Lane 1-3: 1 minute; Lane 4-6: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

IHC

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SSRP1 antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SSRP1 antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

IHC

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SSRP1 antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

计算重置

起始浓度（M）× 起始体积（L）= 最终浓度（M）× 最终体积（L）

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

体积

浓度

体积

计算重置

分子量计算器

Enter the chemical formula of the compound to calculate its molar mass and elemental composition

分子结构式

总分子量

g/mol

计算重置

动物实验计算换算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH₂O，那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 100 μL DMSO （母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法： 取 100 μL DMSO 母液，添加 400 μL PEG300 混匀澄清，再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH₂O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD，玉米油等，均可在诺渊网站点击购买。

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系客服为您提供正确的澄清溶液配方）

% DMSO

% Tween 80

% Saline/PBS/ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系客服）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。