WB
Western blot analysis using Flag tag mouse monoclonal antibody against BSA-Flag and BSA lysates. 12% SDS-PAGE gel. Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane, and block it with TBST plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST, 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST again, 5 minutes each time. Use ECL for luminescence. staining time: 60SIHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Flag tag antibody with DAB staining. Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6. 0) for 2 minutes. Wash the sections with ddH₂O and PBS. Block the tissue with 10% non-immune goat serum at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP, at a ratio of 1:200 and incubate for one hour. Use DAB as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Flag tag antibody with DAB staining. Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6. 0) for 2 minutes. Wash the sections with ddH₂O and PBS. Block the tissue with 10% non-immune goat serum at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP, at a ratio of 1:200 and incubate for one hour. Use DAB as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.ELISA
Blue line:Antibody; Orange line: Isotype control antibody| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Recombinant protein |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 宿主 | Mouse |
| 已测试的应用 | ELISAIHCWB |
| 物种反应性 | All |
| 浓度 | 1mg/ml |
| 纯化 | Affinity purified |
| 蛋白别名 | DDDDK epitope tag antibody DDDK antibody ddk antibody DYKDDDDK antibody DYKDDDDK epitope tag antibody DYKDDDDK tag antibody ECS epitope tag antibody ECS tag antibody Enterokinase Cleavage Site epitope tag antibody Enterokinase Cleavage Site tag antibody FLAG antibody FLAG tag antibody |
| 分子量 | 70kDa |
| 应用稀释比 |
WB:1:1000 IHC:1:1000 |
| 功能 | The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. |
| 亚细胞定位 | Cell membrane Cell membrane Cell membrane |
| 产品形态 | Liquid |
| 缓冲液 | Purified antibody in PBS with 0.05% sodium azide. |
| 保存条件 | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
