| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Polypeptide |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | ELISA |
| 物种反应性 | HumanMouseRat |
| 浓度 | 1mg/ml |
| 纯化 | Protein A |
| 基因名称 | PIK3R1/PIK3R3 |
| 基因别名 | AGM7; GRB1; IMD36; p50alpha; p55alpha; p85; p85-ALPHA; p85alpha; PI3K; PI3KA |
| 基因全称 | phosphoinositide-3-kinase regulatory subunit 1/phosphoinositide-3-kinase regulatory subunit 3 |
| 基因简介 | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]/Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016] |
| 蛋白别名 | growth factor receptor bound 1; p85-alpha; GRB1; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase p85 alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha); phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); PI3 kinase-associated p85; PI3-kinase p85 subunit alpha; PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; PI3K-p46; polypeptide 1 p85 alpha; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; RP4-533D7.2./p55PIK; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase p55 subunit; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; ptdIns-3-kinase p85-gamma; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; RP4-533D7.2 |
| 分子量 | 83kDa/54kDa |
| Uniprot ID | P27986 | Q92569 |
| 应用稀释比 |
ELISA:1:1000-1:10000 |
| 修饰 | Unmodification |
| 标记 | Unconjugated |
| 亚细胞定位 | Cytoplasm/cytosol;phosphatidylinositol 3-kinase complex, class IA;Complete GO annotation on QuickGO |
| 产品形态 | Liquid |
| 缓冲液 | Purified antibody in PBS with 0.05% PC300. |
| 保存条件 | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
