Application
Akt1/2/3 antibody detects Akt1/2/3 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: Akt1 protein stained by Akt1/2/3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody .Application
Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal . Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary. |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | ICC/IFIHCIHC-PIPWB |
| 物种反应性 | HumanMouseRat |
| 浓度 | 1.0mg/ml (please refer to the vial label for the specific concentration) |
| 纯化 | Affinity purified |
| 基因别名 | AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, prot |
| 分子量 | 56 kDa (note) (4) |
| 应用稀释比 | *Optimal dilutions/concentrations should be determined by the researcher. |
| 亚细胞定位 | Cytoplasm , Nucleus , Cell membrane |
| 产品详情 | Rabbit Polyclonal antibody to AKT1+2+3 (v-akt murine thymoma viral oncogene homolog 1)The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
| 产品形态 | Liquid |
| 缓冲液 | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| 保存条件 | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
