Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000Application
Sample (20 ug of whole cell lysate) A: HeLa cell lysate B: HeLa cell transfect with AKT 7.5% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Akt3 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: Rat brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Recombinant protein encompassing a sequence within the center region of human Akt3. The exact sequence is proprietary. |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 已测试的应用 | ICC/IFWB |
| 物种反应性 | HumanMouseRat |
| 浓度 | 0.5mg/ml |
| 纯化 | Affinity purified |
| 基因别名 | DKFZp434N0250 antibody; PKB-GAMMA antibody; PKBG antibody; PRKBG antibody; RAC-PK-gamma antibody; RAC-gamma antibody; STK-2 antibody; AKT3 antibody; PKB gamma antibody; RAC-gamma serine/threonine-protein kinase antibody; RAC-gamma serine/threonine protein |
| 分子量 | 56 kDa (note) (4) |
| 应用稀释比 |
*Optimal dilutions/concentrations should be determined by the researcher.
: |
| 标记 | Unconjugated |
| 亚细胞定位 | Cytoplasm , Membrane |
| 产品详情 | Rabbit Polyclonal antibody to AKT3 (v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma))The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq] |
| 产品形态 | Liquid |
| 缓冲液 | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| 保存条件 | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
