Application
A Peptide dotblot shows RM168 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity. with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2),. or dimethylated Lysine 36 (K36me2).Application
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.Application
RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys18). |
| 克隆性 | Monoclonal |
| 同种型 | IgG |
| 宿主 | Rabbit |
| 克隆号 | RM168 |
| 已测试的应用 | DotELISAWBMultiplexing |
| 物种反应性 | Human |
| 浓度 | 1.0mg/ml (please refer to the vial label for the specific concentration) |
| 纯化 | Affinity purified |
| 基因别名 | H3FA ; histone cluster 1; H3a ; H3/A ; HIST1H3A ; H3K18Me2 antibody |
| 靶标 | Histone H3 (Dimethyl Lys18) |
| 应用稀释比 |
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| 修饰 | Dimethyl Lys18 |
| 标记 | Unconjugated |
| 产品详情 | Rabbit Monoclonal antibody [RM168] to Histone H3 (Dimethyl Lys18)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| 产品形态 | Liquid |
| 缓冲液 | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
| 保存条件 | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
