Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KHDRBS1. |
| 克隆性 | Polyclonal |
| 宿主 | Rabbit |
| 已测试的应用 | ELISAWB |
| 物种反应性 | Human |
| 浓度 | 1.0mg/ml |
| 纯化 | Affinity purified |
| 基因名称 | KHDRBS1 |
| 蛋白别名 | KHDRBS1, FLJ34027, Sam68, p62, p68 |
| 分子量 | 48 kDa |
| Gene ID | 10657 |
| 应用稀释比 | KHDRBS1 antibody can be used for detection of KHDRBS1 by ELISA at 1:12500. KHDRBS1 antibody can be used for detection of KHDRBS1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| 产品详情 | KHDRBS1 recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. KHDRBS1 play a role in G2-M progression in the cell cycle. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.1) Duran, A., (2008) Cancer Cell 13 (4), 343-354. |
| 产品形态 | Liquid |
| 缓冲液 | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
| 保存条件 | For short periods of storage (days) store at 4˚C. For longer periods of storage, store KHDRBS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
