Application
Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Daudi whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining KHDRBS1 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining KHDRBS1 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| 抗体类型 | Primary Antibodies |
|---|---|
| 免疫原 | This KHDRBS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 259-291 amino acids from the Central region of human KHDRBS1. |
| 克隆性 | Polyclonal |
| 同种型 | Ig |
| 宿主 | Rabbit |
| 已测试的应用 | IHC-PWB |
| 物种反应性 | HumanMouse |
| 浓度 | 1.0mg/ml |
| 纯化 | Affinity purified |
| 基因名称 | KHDRBS1 |
| 蛋白别名 | KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68, KHDRBS1 ( HGNC:18116 ) |
| 分子量 | 48 kDa |
| Gene ID | 10657 |
| 应用稀释比 | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 |
| 产品详情 | Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed: 22253824 ).1) Wong G.,et al.Cell 69:551-558(1992). |
| 产品形态 | Liquid |
| 缓冲液 | Supplied in PBS with 0.09% (W/V) sodium azide. |
| 保存条件 | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL， 一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O， 那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 100 μL DMSO （ 母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法： 取 100 μL DMSO 母液， 添加 400 μL PEG300 混匀澄清， 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD， 玉米油等， 均可在诺渊网站点击购买。
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL Saline/PBS/ddH2O，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
