Rabbit anti-SRFBP1 polyclonal antibody - N-terminal region

宿主:
Rabbit
已测试的应用:
WB
物种反应性:
Dog,Horse,Rabbit
货号:TS654147

产品信息

抗体类型 Primary Antibodies
免疫原 The immunogen for anti-SRFBP1 antibody: synthetic peptide directed towards the N terminal of human SRFBP1
克隆性 Polyclonal
同种型 IgG
宿主 Rabbit
已测试的应用 WB
物种反应性 DogHorseRabbit
浓度 1.0mg/ml
纯化 Affinity purified
基因名称 SRFBP1
基因别名 BUD22; FLJ25286; P49; Rlb1; STRAP; p49/STRAP
基因全称 Serum response factor binding protein 1
基因简介 SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. It may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells.
蛋白别名 BUD22; FLJ25286; P49; Rlb1; STRAP; p49/STRAP
分子量 49kDa
序列 429 amino acids
Gene ID 153443
Uniprot ID Q8NEF9
应用稀释比 WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
修饰 Unmodification
标记 Unconjugated
产品形态 Liquid
缓冲液 phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存条件 Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
		Western blot
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate
质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
起始浓度(M)× 起始体积(L)= 最终浓度(M)× 最终体积(L)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
分子量计算器
Enter the chemical formula of the compound to calculate its molar mass and elemental composition
g/mol
动物实验计算换算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:

比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL, 一共给药动物10只,您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O, 那么您的工作液浓度为2 mg/mL

母液配置方法:2 mg 药物溶于 100 μL DMSO ( 母液浓度为 20 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法: 取 100 μL DMSO 母液, 添加 400 μL PEG300 混匀澄清, 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如: DMSO, PEG300, PEG400, Tween 80, SBE-β-CD, 玉米油等, 均可在诺渊网站点击购买。

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系客服为您提供正确的澄清溶液配方)
+
+
+

计算结果:

工作液浓度 mg/ml;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL Saline/PBS/ddH2O,混匀澄清。

1. 首先保证母液是澄清的;
           2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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